Synchronization of gene expression across eukaryotic communities through chemical rhythms
نویسندگان
چکیده
منابع مشابه
Cloning and Expression of VP2 Gene of Infectious Bursal Disease Virus in Eukaryotic Cells
Infectious bursal disease (IBD) is an economically important viral disease of chickens with worldwide distribution which suppresses the immune system of young chickens. VP2 is the major host-protective protein of infectious bursal disease virus (IBDV). The encoding region of VP2 protein was PCR amplified from a plasmid containing a cDNA fragment of large genomic segment of IBDV, strain D78. Thi...
متن کاملCloning and secretory expression of VP2 gene of infectious bursal disease virus in eukaryotic cells
VP2 gene coding region of a vaccinal strain (D78) of infectious bursal disease virus (IBDV) was clonedin a eukaryotic expression vector, pSec Tag2A. The gene was placed downstream of Ig κ chain leadersequence, under the control of human cytomegalovirus (hCMV) immediate early enhancer and promoter. Theconstruct pSec Tag2A-VP2 was transfected in COS-7 cell line and the expression and secretion of...
متن کاملCloning and Expression of Rabies Virus Glycoprotein Gene into Eukaryotic System
Background and Aims: The aim of this study was cloning and expression of rabies virus glycoprotein by a eukaryotic expression plasmid pcDNA3.1(+) in BSR cell line. This construct might be used for a potential DNA vaccine. Materials and Methods: Glycoprotein gene was synthesized and cloned into pBluescript vector and then sub cloned into eukaryotic expression vector (pcDNA3.1(+)). After verifica...
متن کاملCloning and high level expression of bovine interferon gamma gene in eukaryotic cells (COS-7)
Interferon gamma (IFN-γ) is one of the key cytokines in defining T helper 1 lymphocyte immuneresponses. In this study, the bovine IFN-γ gene was cloned from spleen tissue RNA using the reversetranscription-polymerase chain reaction (RT-PCR). IFN-γ cDNA was sub-cloned and expressed inmammalian expression plasmid (pcDNA3.1(+)) under the control of the human cytomegalovirus (CMV)promoter. The pred...
متن کاملStochastic synchronization of circadian rhythms
The models of circadian genetic oscillators involving interwined feedback processes in molecular level genetic network in Drosophila melanogaster and Neurospora crassa are studied and examine mechanisms whereby synchronization can arise in an assembly of cells. The individual subcellular circadian oscillatory processes are stochastic in nature due to small numbers of molecules are involved in t...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nature Communications
سال: 2021
ISSN: 2041-1723
DOI: 10.1038/s41467-021-24325-z